1 Department of Biotechnology, Technical University of Denmark2 Department of Chemical and Biochemical Engineering, Technical University of Denmark3 Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark4 unknown5 Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark
The alleviation of glucose control of maltose metabolism brought about by MIG1 disruption was compared to that by MAL overexpression in a haploid Saccharomyces cerevisiae strain. The sugar consumption profiles during cultivation of the wild type, single transformants and a double transformant in a mixed glucose-maltose medium revealed that the MAL-constitutive strains were more alleviated than the single MIG1-disrupted transformant. While all transformants exhibited higher maximum specific growth rates (0.24-0.25 h(-1)) in glucose-maltose mixtures than the wild type strain (0.20 h(-1)), the MAL-constitutive transformants grew even faster (0.27-0.30 h(-1)) in pure glucose medium than the wild type strain (0.24 h(-1)).
Food Technology and Biotechnology, 1997, Vol 35, Issue 4, p. 287-292