Determination of trace metal ions via on-line separation and preconcentration by means of chelating Sepharose beads in a sequential injection lab-on-valve (SI-LOV) system coupled to electrothermal atomic absorption spectrometric detection
Long, Xiangbao2; Hansen, Elo Harald1; Miró, Manuel4
1 Department of Chemistry, Technical University of Denmark2 unknown3 University of the Balearic Islands4 University of the Balearic Islands
The analytical performance of an on-line sequential injection lab-on-valve (SI-LOV) system using chelating Sepharose beads as sorbent material for the determination of ultra trace levels of Cd(II), Pb(II) and Ni(II) by electrothermal atomic absorption spectrometry (ETAAS) is described and discussed. The samples are adjusted to pH 5.0 on-line in the system for optimum operation. The target ions are adsorbed by chelation on the surface of the beads, contained in a 20 mul microcolumn within the LOV, and following elution by 50 mul 2M nitric acid, the eluate is, as sandwiched by air segments, introduced into the ETAAS. Based on the consumption of 1.8 ml sample solution, retention efficiencies of 95%, 75% and 90%, enrichment factors of 86, 68 and 81, and determination limits of 0.001, 0.07 and 0.02 mug l-1 were obtained for Cd(II), PbII) and Ni(II), respectively. The beads can be used repeatedly for at least 20 times without decrease of performance, yet can be replaced at will if the circumstances should so dictate. The optimized procedural parameters showed that 12 sample h-1 could be prepared and successfully analyzed. The results obtained for three standard reference materials agreed very well with the certified values.