Nielsen, Jakob Blæsbjerg6; Klejnstrup, Marie Louise1; Khorsand-Jamal, Paiman1; Holm, Dorte Koefoed1; Nielsen, Michael Lynge7; Kabat, Anna Monika8; Rank, Christian1; Gotfredsen, Charlotte Held5; Larsen, T. O.9; Mortensen, Uffe Hasbro6
1 Department of Systems Biology, Technical University of Denmark2 Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark3 Organic Chemistry, Department of Chemistry, Technical University of Denmark4 Center for Systems Microbiology, Department of Systems Biology, Technical University of Denmark5 Department of Chemistry, Technical University of Denmark6 Eukaryotic Molecular Cell Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark7 Department of Biotechnology, Technical University of Denmark8 Metabolic Signaling and Regulation, Department of Biotechnology and Biomedicine, Technical University of Denmark9 Natural Product Discovery, Department of Biotechnology and Biomedicine, Technical University of Denmark
With the aim of mapping the polyketome of A. nidulans we have made a library of strains, which individually overexpress PKS genes from an ectopic locus. A screen of this collection on different media demonstrated that AN6448 leads to production of 3-MOA. An inspection of the DNA sequence surrounding this gene uncovered a putative gene cluster including a gene, AN6446, with homology to transcription factors. Based on this observation, we decided to overexpress AN6446. A qRT-PCR analysis of this strain was used to delineate the borders of the gene cluster as well as to stimulate formation of cichorine and a number of new products, from the gene cluster. Subsequent deletion of all genes allowed several steps in the biosynthetic pathway in this cluster to be clarified.
Planta Medica, 2012, Vol 78, Issue 11, p. 1113-1113
Main Research Area:
International Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF, 2012