1 Department of Systems Biology, Technical University of Denmark2 Department of Biochemistry and Nutrition, Technical University of Denmark
The incorporation of vaccenic acid (VA, 0.5 and 1.2%), conjugated linoleic acid (CLA, mixture of primarily c9,t11- and t10,c12-CLA, 1.2%), linoleic acid (LA, 1.2%) and oleic acid (OA, 1.2%) into different tissues of mice was examined. The effects on the fatty acid composition of triacylglycerols (TAG) and phospholipids (PL) in kidney, spleen, liver and adipose tissue were investigated. VA and CLA (c9,t11- and t10,c12-CLA) were primarily found in TAG, especially in kidney and adipose tissue, respectively. Conversion of VA to c9,t11-CLA was indicated by our results, as both fatty acids were incorporated into all the analyzed tissues when a diet containing VA but not c9,t11-CLA was fed. Most of the observed effects on the fatty acid profiles were seen in the CLA group, whereas only minor effects were observed in the VA groups compared with the CA group. Thus, CLA increased n-3 polyunsaturated fatty acids (PUFA) in PL from kidney and spleen and lowered the ratio of n-6/n-3 PUFA in these tissues. Furthermore, CLA increased C-22 PUFA in the PL fraction of kidney, spleen and liver, but reduced the level of arachiclonic acid in PL of liver and spleen and lowered the Delta(9)-desaturation indexes in all analyzed tissue TAG.
European Journal of Lipid Science and Technology, 2006, Vol 108, Issue 6, p. 468-478