1 Faculty of Science, SDU2 Department of Biochemistry and Molecular Biology, Faculty of Science, SDU3 Department of Biochemistry and Molecular Biology, Faculty of Science, SDU
Electrospray ionization and matrix assisted laser desorption/ionization are effective ionization methods for mass spectrometry of biomolecules. Here we describe the capabilities of these methods for peptide and protein characterization in biotechnology. An integrated analytical strategy is presented encompassing protein characterization prior to and after cloning of the corresponding gene.
Nature Biotechnology, 1996, Vol 14, Issue 4, p. 449-57
Biotechnology; Mass Spectrometry; Molecular Weight; Oligonucleotide Probes; Peptide Mapping; Protein Conformation; Recombinant Proteins; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization