This thesis covers three main results obtained during the project: A reliable method of performing serial sectioning on metal samples utilizing a Logitech polishing machine has been developed. Serial sectioning has been performed on metal samples in 1 µmsteps utilizing mechanical polishing, and in 2 µm steps when electrochemical polishing was needed. A method by which reliable EBSP line scans may be performed by scanning three parallel lines has been developed. This method allows lines of the order of 1cm in length to be characterized with a 1 µm or better spatial resolution. The method is proven to be a good way of determining microstructural parameters, which are important when studying recrystallization dynamics. The nucleation of recrystallizationat triple junctions has been studied by 3 dimensional X-ray diffraction (3DXRD), allowing for the first time the deformed and recrystallized microstructures to be compared at a given nucleation site in the bulk of a metal sample. From an experiment threenuclei were identified, their respective crystal orientations were determined, and growth curves were obtained for two of them.