Ravn, Helle Christine1; Damstrup, Marianne L.3; Meyer, Anne S.1
1 Department of Chemical and Biochemical Engineering, Technical University of Denmark2 Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark3 Novozymes A/S
Enzymatic conversion of fish oil free fatty acids (FFA) or fatty acid ethyl esters (FAE) into glycerides via esterification or transesterification was examined. The reactions catalyzed by Lipozyme™ 435, a Candida antarctica lipase, were optimized. Influence on conversion yields of fatty acid chain length, saturation degree, temperature, enzyme dosage, molar ratio glycerol:fatty acids, acyl source composition (w/w ratio FFA:FAE), and reaction time was evaluated collectively by multiple linear regression. All reaction variables influenced the conversion into glycerides. Transesterification of FAE produced the highest yields of 94–95% (w/w) conversion yield at 1:3 glycerol:FAE, 25h, 66°C, enzyme dosage 3.1%w/w.
Biocatalysis and Agricultural Biotechnology, 2012, Vol 1, Issue 4, p. 273-279
Immobilized Candida antarctica lipase B; Fish oil concentrates; Omega-3 fatty acids; Central composite design; Esterification; Transesterification