Kristiansen, T B2; Knudsen, T B2; Ohlendorff, S2; Eugen-Olsen, J1
1 Clinical Research Centre, Amager and Hvidovre Hospital, The Capital Region of Denmark2 unknown
The CCR5 Delta32, CCR2 64I, SDF1 3'A, and CCR5 promoter 59029 polymorphisms have been suggested to influence HIV-1 disease progression. Furthermore, the CCR5 Delta32 and the CCR2 64I polymorphisms have been associated with various other diseases. The purpose of the present study was to develop a multiplex assay for the simultaneous determination of these four polymorphisms. Results obtained with the multiplex assay were compared to results obtained by conventional RFLP-PCR and no differences were observed. The multiplex assay offers a quick tool for the determination of the CCR5 Delta32, CCR2 64I, SDF1 3'A, and CCR5 promoter 59029 A/G polymorphisms.
Journal of Immunological Methods, 2001, Vol 252, Issue 1-2, p. 147-51