Pedersen, Mads2; Lauritzen, Henrik Klitgaard5; Frisvad, Jens Christian6; Meyer, Anne Boye Strunge2
1 Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark2 Department of Chemical and Biochemical Engineering, Technical University of Denmark3 Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark4 Department of Systems Biology, Technical University of Denmark5 unknown6 Fungal Chemodiversity, Department of Biotechnology and Biomedicine, Technical University of Denmark
Twenty Aspergillus strains were evaluated for production of extracellular cellulolytic and xylanolytic activities. Aspergillus brasiliensis, A. niger and A. japonicus produced the highest xylanase activities with the A. brasiliensis and A. niger strains producing thermostable beta-xylosidases. The beta-xylosidase activities of the A. brasiliensis and A. niger strains had similar temperature and pH optima at 75 degrees C and pH 5 and retained 62% and 99%, respectively, of these activities over 1 h at 60 degrees C. At 75 degrees C, these values were 38 and 44%, respectively. Whereas A. niger is a well known enzyme producer, this is the first report of xylanase and thermostable beta-xylosidase production from the newly identified, non-ochratoxin-producing species A. brasiliensis.
Biotechnology Letters, 2007, Vol 29, Issue 5, p. 743-748