Young, J F2; Bertram, H C3; Theil, P K3; Petersen, A-G D3; Poulsen, K A3; Rasmussen, M3; Malmendal, Anders5; Nielsen, N C3; Vestergaard, M3; Oksbjerg, N3
1 Section of Cellular and Metabolic Research, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, Københavns Universitet2 Danish Institute of Agricultural Sciences, Department of Food Science, Research Centre Foulum, Tjele, Denmark.3 unknown4 Section VI. Building 18.4, Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, Københavns Universitet5 Section VI. Building 18.4, Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, Københavns Universitet
Duroc and Landrace pigs as well as primary myotubes from these breeds were used to investigate mechanisms behind differences in their response to creatine monohydrate (CMH). Pigs were supplemented with 0, 12.5, 25 or 50g CMH/d for 5 days (n=10 per treatment and breed). Plasma levels of creatine increased dose-dependently in both breeds, while muscle-creatine phosphate content increased only in the Duroc pigs. (1)H NMR metabolic profiling showed a tendency towards clustering according to CMH supplementation only among Duroc pigs, revealing a stronger response compared to Landrace pigs. The abundance of insulin-like growth factor I and myostatin mRNA was decreased by CMH supplementation while that of type 1 IGF-receptor and creatine transporter was unaffected. Protein synthesis, increased in the myotubes from both breeds, indicating protein accretion, but no effect was observed on the mRNA abundance of IGF-I, type 1 IGF-receptor, myostatin or the creatine transporter in myotubes.