Olsson, Lisbeth3; Sørensen, H. R.4; Dam, B. P4; Krogh, Kristian Bertel Rømer Mørkeberg2; Meyer, Anne Boye Strunge5
1 Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark2 Department of Systems Biology, Technical University of Denmark3 Department of Biotechnology, Technical University of Denmark4 unknown5 Department of Chemical and Biochemical Engineering, Technical University of Denmark
Fermentations with three different xylose-utilizing recombinant Saccharomyces cerevisiae strains (F12, CR4, and CB4) were performed using two different wheat hemicellulose substrates, unfermented starch free fibers, and an industrial ethanol fermentation residue, vinasse. With CR4 and F12, the maximum ethanol concentrations obtained were 4.3 and 4 g/L, respectively, but F12 converted xylose 15% faster than CR4 during the first 24 h. The comparison of separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) with F12 showed that the highest, maximum ethanol concentrations were obtained with SSF. In general, the volumetric ethanol productivity was initially, highest in the SHF, but the overall volumetric ethanol productivity ended up being maximal in the SSF, at 0.013 and 0.010 g/Lh, with starch free fibers and vinasse, respectively.
Applied Biochemistry and Biotechnology, 2006, Vol 129, Issue 1-3, p. 117-129