1 National Food Institute, Technical University of Denmark2 Microbial Ecology, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark3 Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark4 National Veterinary Institute, Technical University of Denmark5 Division of Microbiology and Risk Assessment, National Food Institute, Technical University of Denmark6 National Institute of Aquatic Resources, Technical University of Denmark7 Technical University of Denmark8 Royal Veterinary and Agricultural University9 Copenhagen Center for Health Technology, Center, Technical University of Denmark
effects of time, host and gender
Denaturing gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (T-RFLP) and plating on selective agars were used to study variation in the fecal microbiota of rats over time as well as variation between individuals. Investigated rats were either conventional and specific pathogen free (SPF). or human flora associated (HFA). A higher variation (p <0.05) in fecal microbiota over time was observed for HFA than for SPF animals. Analysis of DGGE and T-RFLP profiles of fecal microbiota from SPF and HFA rats revealed that variation over time was less significant than variation between individuals, and that phylogenetic profiles clustered according to gender. These observations should be taken into account when designing future research addressing changes in fecal microbiota.
Journal of Microbiological Methods, 2006, Vol 66, Issue 1, p. 87-95
LIFE; DGGE; T-RFLP; Fecal microbiota; Community analysis; DGGE; community analysis; T-RFLP; fecal microbiota