1 Department of Micro- and Nanotechnology, Technical University of Denmark2 Section of Poultry Diseases, Division of Poultry, Fish and Fur Animals, National Veterinary Institute, Technical University of Denmark3 Division of Poultry, Fish and Fur Animals, National Veterinary Institute, Technical University of Denmark4 National Veterinary Institute, Technical University of Denmark5 National Food Institute, Technical University of Denmark6 unknown7 Others, Technical University of Denmark
A DNA microarray for detection of Campylobacter spp. was recently developed and applied to detect Campylobacter spp. directly from chicken feces. Sixty-five pooled chicken cloacal swab samples from 650 individual broiler chickens were included in the study. The results of Campylobacter sp. detection obtained with DNA microarrays were compared to those obtained by conventional culture and gel electrophoresis. By conventional culture, 60% of the samples were positive for either Campylobacter jejuni or Campylobacter coli. By PCR and capillary electrophoresis, 95% of the samples were positive for Campylobacter spp., whereas with DNA microarrays all samples were positive for Campylobacter spp. By application of DNA microarray analysis, the isolates in 4 samples (6%) could not be identified to the species level, whereas by PCR-capillary electrophoresis, the isolates in 12 samples (19%) remained unidentified. Interestingly, PCR-capillary electrophoresis analysis revealed that two (3%) of the samples were positive for both C. jejuni and C. coli, while DNA microarray analysis revealed that nine (14%) of the samples were positive for both species. Of 65 samples, 2 samples were identified to contain C. coli by conventional culture but were positive for C. jejuni by both PCR-capillary electrophoresis and DNA microarray analysis. The discrepancy between the methods is discussed.
Journal of Clinical Microbiology, 2004, Vol 42, Issue 9, p. 3985-3991
Animals; Base Sequence; Campylobacter coli; Campylobacter jejuni; Chickens; DNA Primers; Feces; Oligonucleotide Array Sequence Analysis; Polymerase Chain Reaction; Specimen Handling;